Musical Controversy Essays - Music, Eminem, , Term Papers

Musical Controversy There is a kind of music, which is commonly referred to as ?Pop? music. It attracts a variety of Americans of very different geographical, racial, and economical backgrounds. Some of the most popular artists these days that are considered ?Pop? are N'Sync, Britney Spears (who, having recently turned eighteen, doesn't seem to mind letting the guys know that she's legal now), Cristina Aguilera, Eminem, Limp Bizkit, and Tupac Shakur. It would take a thousand pages to describe the entire commercial and cultural aspects of the music industry, so I will talk about the hip-hop community. Some hip-hop is commonly referred to as ?Pop? music, simply because it is popular with a wide variety of crowds. I intend to show you how the different types of crowds under the Pop category interact with one another, especially at popular awards ceremonies, such as the recent MTV Video Music Awards '00. MTV's largest show and party of every year is their Video Music Awards, celebrated in 1999 on 9/9/99. In 2000, it was highlighted by performances by Eminem (aka Marshall Mathers), N'Sync, a side of Britney Spears which brought a chuckle from a few early Madonna haters, and others. In an interview with reporters before the show, 26 year-old white rapper Eminem stated ?It ain't often you get so many people that I don't like into one room together.? Eminem's relationship with the music industry is a strange one ? you either love him or you hate him. He openly hates gays, women, and children. He is being petitioned to be arrested by a national gay rights activist group for his homophobic lyrics and attitude. So, you ask, why is he such a loved character if he is so ?shady His most recent album, The Marshall Mathers LP, sold 1.7 million copies its first week ? the most for any single artist in history. Also notable for comment is that he achieved this at a time when illegal music piracy is so commonly practiced that the government cannot even begin to regulate it. This is another issue however. The recent media chase over Shawn Fanning, an innocent-looking 19 year-old college dropout, is amazing for one who has not been along for the ride the whole time. Shawn Fanning released Napster in 1999, it was one of the top 10 most quickly downloaded programs of 1999. It's ability to ?share? music files, called mp3s, digitally over the Internet made it become absolutely essential for anyone between the ages of 12-24 to have. Gone were the days when you had to listen to the radio or buy a single ? now you could just go online, wait anywhere from two to thirty minutes, and have the song that you wanted, for play on your own computer or to burn to a blank CD. In steps ancient rock legends, Metallica. Metallica drummer, Lars Ulrich, took a stand against Napster. He claims that it is violating copyright laws set by the United States. Fanning's creation, by allowing fans to disperse their music to one another, is equivalent to stealing right from the band. In a sense, he is correct. In re ality, if it weren't for the popularity that the program has already achieved, it would have been shut down long ago without a multi-million dollar court case. Now it becomes just one of the many media-hyped incidents in the music industry. The pop music industry is a complicated web of underground culture. Because America is such a diverse nation, the music industry reflects the diversity of the country. From white rappers to thirteen year-old black rappers, to seventy year old country singers, there is definitely a type of music that most people enjoy. Bibliography: Marshall Mathers, spoken in pre-show interview before MTV VMA 2000

Blood type b in asian countries Essays

Blood type b in asian countries Essays Blood type b in asian countries Paper Blood type b in asian countries Paper 2001). These variants harbored missense mutations in specific exons that generate alterations in the carbohydrate chain of the ABO antigens. Such reports have significantly affected serological protocols that were closely associated with donor-patient compatibility testings, because these variants had the capacity to express the antigens at a broader range of degree of expression than what was classically described. This dilemma may cause mistyping of blood groups which, in turn, may affect transfusion and transplantation procedures. The ABO blood group system has been determined to follow a unique geographic distribution (Fukumori et al. , 1996). Frequencies of individuals with blood type B are known to be particularly high in Asian countries, ranging up to 30% is specific populations such as the Himalayans. The Himalayan region has been reported to have a history of smallpox and bubonic plague epidemics, which are specific medical illnesses that confer particular physiological responses in the body that blood types A and O are selected against (Yamamoto et al. , 1993). Such selection is also associated with the development of resistance to the other antigens, hence blood type B is said to be fixed in the area through the mechanism of genetic drift. Investigations on the ABO locus has revealed that random sequence variations occur within this region, resulting in substitutions of nucleotides bases that are presumably less susceptible to selection pressure, hence it is possible to trace the phylogeny of the B alleles in a more robust way. The diversification of the B locus may be a result of two genetic mechanisms. Point mutations may have occurred in the consensus ABO locus due to recombination events (Olsson and Chester, 1995). The fixation rates of a mutation may be calculated based on its extent of homology to the consensus B locus. This may be observed in the high frequencies in Asian populations and the low frequencies in different white populations. Recombination facilitates the generation of new variants of blood type B may also be enhanced by inter-lineage sequence transfer, resulting in intermediate products of the B allele. Blood type B is strongly associated with specific medical diseases such as esophageal carcinoma, infantile diarrhea and typhoid fever (Su et al. , 2001). These associations may be due to the selection against the other blood types during the onset of the medical illness. In addition, the B antigen is maximally expressed in these populations because the physiological settings of these individuals are optimal for the fixation of the blood type B and its related subtypes, as well as the increased suppression of expression of the A antigen in the red blood cells. The ABO blood typing system should therefore be cautiously analyzed especially when dealing with serology, transfusion and transplantation procedures, together with the ethnicity data that is gather from the patient, in order to avoid mistyping and adverse rejection reactions. References Fukumori Y, Ohnoki S, Shibata H, Nishimukai H (1996): Suballeles of the ABO blood group system in a Japanese population. Hum. Hered. 46:85-91. Olsson ML, Chester MA (1995): A rapid and simple ABO genotype screening method using a novel B/O2 versus A/O1 discriminating nucleotide substitution at the ABO locus. Vox Sang. 69:242-247. Olsson ML, Irshaid NM, Hosseini-Maaf B, Hellberg A, Moulds MK, Hannele Sareneva, Chester MA (2001): Genomic analysis of clinical samples with serologicABO blood grouping discrepancies: identification of 15 novel A and B subgroup alleles. Blood 98:1585-1593. Su M, Lu SM, Tian DP, Zhao H, Li XY, Li DR, Zheng ZC (2001): Relationship between ABO blood groups and carcinoma of esophagus and cardia in Chaoshan inhabitants of China. World J. Gastroenterol. 7(5):657-61. Yamamoto F, McNeill PD, Yamamoto M, Hakomori S, Bromilow IM, Duguid JK (1993): Molecular genetic analysis of the ABO blood group system, 4:another type of O allele. Vox Sang. 64:175-178.

Mastering Time Management Essay Example | Topics and Well Written Essays - 750 words - 1

Mastering Time Management - Essay Example How an individual allocates time for different activities depends upon his level of education and understanding of life. As a manager one must first know how to manage oneself and only then can one manage an organization or a subordinate. One has to identify what is important in life and spend more time on that. If one knows of the hectic week ahead, decisions and planning in advance helps (Green & Skinner, 2005). Prioritizing work reveals that some work could be delegated or were not really important. It can help to avoid stress that reduces efficiency. Stress also leads to high absenteeism and labor turnover, all of which can be measured in terms of money. Time management can help to avoid missing deadlines which hampers customer relations, affects the image and competitiveness of the firm. it helps to develop cognitive skills. It enables a person to be more organized, assertive, he is able to prioritize work and achieve targets. The whole environment is less stressful and results in better health conditions of the employees. In the field of nursing time was a condition that structured the planning, accomplishment and result of nurses’ work (Bowers, Lauring & Jacobson, 2000). Their main source of job dissatisfaction was too little time. Limited time makes it difficult to complete the required work and it is difficult to spend time with residents. Timing is important even in political campaigns and decisions. It can mar the campaigns if some events in the nations erupt just during that period. Timing makes or breaks the situation; timing influences the victory or loss as in the case of Rudy Giulani who faced bad news from the drug indictment of his South Carolina chairman to criticism for skipping meetings of the Iraq Study Group (MSNBC, 2007). The timing could not have been worse as the string of events came just as national polls showed him ahead of his rivals. People want to ‘manage stress’. Managing

Edgar Allen Poe Research Paper Example | Topics and Well Written Essays - 1000 words

Edgar Allen Poe - Research Paper Example Edgar Allan Poe was born in Boston, Massachusetts on January 19, 1809 (Kennedy, 2001, 19). Personal misfortunes were, sadly, a persistent episode during his lifetime. He never met his father who abandoned his mother. His mother died from tuberculosis. After his parents’ death Edgar was adopted by Frances and John Allan, a rich trader in Richmond, Virginia (Kennedy, 2001). From the very beginning of Poe’s authorial vocation, he adored creating verses for the most important persons in his life. Soon after, when he reached maturity and understood life’s unpleasant realities, his narratives became more disquieting and darker, possibly because of his overindulgence in alcohol and drugs (Magistrale, 2001). His horror tales are still regarded as one of the most chilling and frightening tales ever written, and, due to this, several literary scholars contemplated on the possible sources of these dark themes. Numerous literature activists and historians have assumed that h is problematic love life was the root whereas others have pointed to his drug and alcohol addiction (Magistrale, 2001). ... Poe implanted this in his famed masterpiece Alone (Hoffman, 1998, 38): â€Å"From childhood’s hour, I have not been as others were; I have not seen as others saw; I could not bring my passions from a common spring.† As mentioned above, several of the most significant persons in his life were a vast source of influence because of their deaths. His reaction to death consistently included more heightened addiction to alcohol and drugs (Kennedy, 2001); hence, it is difficult to make a decisive difference between the influences of Poe’s individual loved ones. Nevertheless, since the deaths happened prior to the drugs and alcohol addiction, it was only death which occupied the utmost position in his life, bringing about series of self-destruction that sooner or later put an end on his own existence (Kennedy, 2001). In spite of his personal misfortunes, nevertheless, he stays as one of the most treasured and broadly celebrated of all American authors. His disturbing and frightening tales and poems will survive through ages and be read by innumerable people from various societies and different eras, a reality which would have certainly given some spring of calm for this agonized, gifted, and disturbed individual. Almost every well-known author that came after Poe, embodying practically all national literatures, was familiar with him, and writers who were not personally influenced by his idea were compelled somehow to recognize it, if simply in a feat of denial (Kennedy, 2001). The reputation and significance of Poe as an author were at first freed from the nasty rumors of Rufus Griswold, his literary architect, by the determined attempts of Charles Baudelaire, a French writer (Magistrale, 2001). The influence of Poe on 19th century French literature

Public Relations Project Essay Example | Topics and Well Written Essays - 500 words

Public Relations Project - Essay Example is necessary to develop drug abuse prevention awareness among the use, so that students can identify the dangers and impacts of drug abuse in their health and success. As the Public Relations staffer, I was hired by a non-profit organization to develop a broad communication plan in support of working to reduce smoking, especially among high school students, through our group, Stamp Out Smoking. The development of the communication plan usually starts with the assessment of smoking and other adolescent problems, which include examining the level of community risk factors, and the level of smoking in the community (Crano & Burgoon 145). The assessment results are used to raise community awareness of the seriousness and nature of smoking problem, and this information is used to determine the best program that is relevant to the students’ needs. The next step is the analysis of the student’s readiness for prevention. This helps in identifying further steps that are required to lecture the students before the beginning of the prevention effort. The final step involves holding meetings with teachers and student representatives to help implement and maintain research-based programs (Crano & Burgoon 145). This requires resource development for management and staffing with the existing delivery systems. This plan identifies information dissemination as the most effective communication method for creating drug prevention awareness among the high school students. This approach attempts to communicate the dangers of drug abuse by using a fear-arousal technique designs to frighten individuals and attract attention in to not using drugs, including the dangerous impacts of drug abuse on an individual’s health (Crano & Burgoon 259). The information approaches involve classroom lectures about the negative impacts of smoking, including printed materials, short films, and educational pamphlets, which imparts information to the youths about the dangers of smoking. This

Aspergillus Fumigatus Identification and Molecular Character

Aspergillus Fumigatus Identification and Molecular Character IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF Aspergillus fumigatus FROM SOIL R. V. Shalini, and Dr. K. Amutha ABSTRACT: Soil was collected, serially diluted and pure culture obtained; slant was prepared in potato dextrose agar and maintained throughout the study. Morphological, microscopical and macroscopically identification were carried out on the isolated organism. DNA was isolated from the 24 hour culture, for ITS-PCR amplification. DNA was amplified by mixing the template DNA (50nm) with the polymerase reaction buffer, dNTP mix, primers and Taq. Polymerase chain reaction (PCR) was performed in a total volume of 50Â µL reaction mixture. The PCR product was mixed with loading buffer (8Â µL) containing 0.025% bromophenol blue, 40% w/v sucrose in water and then loaded in 2% agarose gel with 0.1% of ethidium bromide and the amplified product was visualized under a UV trans illuminator for further examination. The PCR products were finally sequenced using the help of an automated DNA sequencer at progen Ltd (Salem, India) and analyzed with the BLAST program provided by the National Center f or Bio-technology information (NCBI) to confirm the fungal species. The current study demonstrates that DNA genome containing 18S rRNA has a high degree of analytical sensitivity and specificity (100%) for the detection of a wide range of fungi. OBJECTIVE: To isolate, identify and characterize Aspergillus fumigatus using molecular biological methods. MATERIALS AND METHODS: The soil was collected from different places, pooled together allowed to be dried at room temperature. The morphology based identification of Aspergillus was done which includes the size, shape, colour, ornamentation of spore and mode of attachment. Unfortunately a lot of difficulties arose for phenotypical identification of this fungus due to its unstable characteristics. Comparatively a DNA sequence-based identification format appeared to be the most promising in terms of its speed, ease, objectivity and reliability for species identification. RESULTS: The preliminary morphology based studies showed the isolated fungi as a species of aspergillus.However after the DNA isolation followed by sequencing it was concluded that the particular species identified as Aspergillus was Aspergillus fumigatus. KEY WORDS: Aspergillus, serial dilution, DNA, Sequenced. INTRODUCTION: The presence of organic matter in the soil affects the quantity and quality of microbes in the soil. The development of micro fungi in the soil is favoured by soils having acidic reaction and aerobic condition which is likely present in the soil. However the amount of degradation in the soil is brought about by the organisms present in the soil. 1The rate at which the organic matter is decomposed is inter related with soil microbes. (Arunachalam et al., 1997). Microorganisms come in various sizes and shapes and is determined by the soil ph., temperature, available moisture, degree of aeration, availability of nutrients in the soil etc. The genus of spore forming fungi is found worldwide out of which Aspergillus is the most dominant species and is ubiquitoes.Out of that 95% is occupied by Aspergillus fumigatus. The other pathogenic forms of Aspergillus species are Aspergillus flavus, Aspergillus niger, Aspergillus nidulans, Aspergillus terreus etc. This fungi exists only in mycelial f orm, and is thermo tolerant capable of growing at temperatures between 15-53Â °c.Being a spore producing fungi the spores gets dispersed by wind in the atmosphere. 2Aspergillus fumigatus is the most common among all the airborne saprophytic fungal pathogens in immune compromised patients mostly in developed countries (Latge, 1999). It is the main pathogenic agent of various diseases caused in humans including invasive pulmonary aspergillosis, aspergilloma and allergic bronchopulmonary aspergillosis (Tomee van der Werf, 2001) – the former is a frequent cause death in immune compromised patients. The possession of different virulent traits gives A. fumigatus the ability to cause these diseases. It is a known fact that other members of the genus Aspergillus are either less pathogenic or non- pathogenic. Identification of the most common and important species remains problematic due to the variability in the phenotypic characters. However a validated and a careful approach of phenotypic classification (taxonomy) together with phylogenetic treatment of DNA sequence data is a prerequisite for a reliable and a rapid identification. In our inve stigation we used the molecular techniques (sequencing) for the reliable identification rather the identification based on their microscopic and few physiological features. MATERIALS METHODS: Collection of soil samples: Soil samples were collected from different places (in and around Chengalpattu). The surface deposits were removed to a depth of about 10 cm and the exposed soil was collected to a depth of 2-3cm. The collected soil samples were stored in zip locked covers stored in refrigerator temperature for further analysis. The collected soil samples were passed through a sieve to remove the stones and other impurities. Isolation of fungi: The glass wares were sterilized in an autoclave to a temperature of 120Â °c for twenty minutes. The chemicals were of analytical grade (Himedia). The method used for the isolation of fungi from soil was serial dilution method. 1 gm of soil was weighed and mixed in 10ml of double distilled sterile water. This was used for preparing serial dilutions. 1 ml of the final dilution (10-6 ) was pipetted into the prepared potato dextrose agar media (PDA) amended with a suitable antibiotic Chloramphenicol (12mg/100ml). The plates were incubated at 30Â °c for about seven days. Fungi that appeared on petriplates were isolated. The isolates were picked up based on apparent dissimilarity of cultural characteristics and purified. The purified isolates were identified according to the genera on the basis of cultural characteristics such as nature of growth, spore colour, and pigment production, and on morphological characteristics of mycelia and fruiting bodies (Domsch etal., 1980; Raper and Fenne ll 1965) and maintained in agar slants for future use3. Isolation of DNA: Genomic DNA was extracted from 24 hour old culture. Measured 100 micro gram of mycelium into a sterile 1.5- micro centrifuge tube. Simultaneously ground 1 microgram of dried (vacuum filter mycelium first) in a mortar and pestle treated with liquid nitrogen 5-6 times. Poured the frozen powder into the Eppendorf tube. Added 660 750 Â µl of lysis buffer and 10 Â µl of B-mercaptor.Vortexed the mixture for a few seconds. And Incubated at 65Â °C for 1 hour. Used a water bath for incubation. Centrifuged at a speed of 3400 rpm for 5 minutes at room temperature and aspirated out the top layer.Transfered the top aqueous layer into a fresh Eppendorf tube discarded the bottom layer. Measured out 700 Â µl of chloroform, isoamyl alcohol (24:1) into Eppendorf tube and adjusted the volume to meet a 1:1 ratio of aqueous phase.Vortexed the mixture for a few seconds. Centrifuged at a speed of 12000 rpm for 10 minutes at room temperature and aspirated out 550 600 Â µl of the top layer. Transfered the top aqueous layer into Eppendorf tube and discarded the bottom layer. Added 0.1volume of 3m potassium acetate and 0.7 volume of isopropanol. Mixed well by inverting the tube not by vortexing.Centrifuged for about 10 minutes and discarded the supernatant. Added 0.5 mL of ice cold ethanol (70% and inverted the tube gently, again it was centrifuged for about 5 minutes in a spinner) finally the pellets were resuspended in 100Â µl of TE buffer (PH-8). After further purification DNA was quantified spectrophotpmetrically and the quality was analyzed in 0.9% agorose gel. Amplification of 18srRNA by PCR: For ITS-PCR amplification, DNA was amplified by mixing the template DNA (50nm) with the polymerase reaction buffer, dNTP mix, primers and Taq polymerase chain reaction (PCR) was performed in a total volume of 50Â µL reaction mixture containing Primer (2Â µM/Â µL) 8.0Â µL 10X Buffer 5.0 Â µL 2mM dNTP Mix 5.0Â µL Taq DNA polymerase (5U/Â µL) 0.5Â µL Template DNA (50ng) 2.0Â µL Sterile distilled water 29.5Â µL Total volume 50.0Â µL PCR amplification condition: Amplification was carried out in a primus advanced gradient thermocycler. The PCR was programmed with an initial denaturing at 94Â °C for 5 min, followed by 30 cycles of denaturation at 94Â °c for 30 seconds, annealing at 61Â °c for 30 seconds, and extension at 70Â °c for 2 minutes and a final extension at 72Â °c for 7 minutes. The PCR product was mixed with loading buffer (8Â µL) containing 0.025% bromophenol blue, 40% w/v sucrose in water and then loaded in 2% agarose gel with 0.1% of ethidium bromide and the amplified product was visualized under a UV trans illuminator for further examination. (Sequencing) Sequencing of ITS region for identification of isolated fungi : Chosen Samples of the genomic DNA containing 18S rRNA were shortlisted for more specific species confirmation by using DNA sequencing. The sequenced PCR product was aligned with other isolate sequences from NCBI genbank for identification. The PCR products were finally sequenced using the help of an automated DNA sequencer at progen Ltd (Salem, India) and analyzed with the BLAST program provided by the National Center for Bio-technology information (NCBI) to confirm the fungal species. RESULTS: Macroscopic and Microscopic Analysis: Analysis of the isolated Aspergillus species showed variation in the colony colours, texture, and reverse side colours (table 1 and 2). The morphological microscopic and molecular characteristics showed that the isolate is Aspergillus fumigatus (details given in table 1and 2). Morphological characters of colony (table1) Characteristics Aspergillus fumigatus Surface colour Margins Reverse side Growth Green to dark green Entire Yellow Rapid Microscopic characteristics (table2) Characteristics Aspergillus fumigatus Hyphae Branched septate Conidiophore Present Vesicle Dome shaped Conidia Present Phialides Uniseriate Fruiting body Cleistothecia Fig A1 Fig A2 Morphological characterization of Aspergillus species on potato dextrose agar A1-Aspergillus fumigatus surface colour, A2-Reverse side of the colony. DNA sequencing of ITS region for identification of species: The species of fungi from the PCR sample was identified by DNA sequencing of the internally transcribed spacer (ITS) region of rRNA gene. Segments of the entire ITS regions, including partial 5.8S rRNA and internal transcribed spacer 2, complete sequence, 28S rRNA,partial sequence were amplified using the primer PGF04 5’-GGC ATC GGC C-3’. Amplification of the ITS region of strain Aspergillus fumigatus had a size of 1703bp. It was submitted to the NCBI and the accession number KC 119199 was received. M 1 2 Fig A3 represent the banding pattern of Aspergillus fumigatus from PCR reactions Lane M= Marker, Lane 1= Aspergillus fumigatus, lane 2=Aspergillus fumigatus. DISCUSSION: Detection of A. fumigatus is of great concern because it is a dangerous allergen associated with aspergillosis 5(Abraca et al., 1994; Schuster et al., 2002; Noonimabcet al.2009; Edwin et al., 2010; Gautam et al., 2011). This highlights the importance of correct identification and taxonomical differentiation between different species of Aspergillus. The taxonomy of Aspergillus has always been complex due to its great number of species (nearly 250), which have very few differences. The identification of different Aspergillus species, on the basis of their morphological characters (example, colony colours, and reverse side) is one of the oldest and most adopted methods. Some of the species of Aspergillus have the same morphological features which make it difficult to distinguish between them it is also a time consuming process and may not be accurate (Klich and Pitt, 1988; Samson et al., 2004)6. This shows that morphological and microscopical characters are not enough for fungal identification and it renders the need of molecular techniques for correct species identification. Molecular characterization on the other hand, is a rapid and a quick procedure which requires minimal handling of pathogens. It also helps in distinguishing morphologically, similar fungal species. Several similar studies on the application of PCR technology were used for the identification and detection of fungi, by using internal transcribed spacer (ITS) were already been studied and published7 by several scientist (Henson and French, 1993; Marek et al., 2003; Haughland et al., 2004; Druzhinina etal., 2005).. Many more such studies were also carried out very recently by God et and Munaut (2010) in the differentiation of Aspergillus flavus, A.parasiticus, A.tamarii and A.nomius by PCR-RAPD markers. Similarly, Leema et al. (2010) confirmed the species A. flavus by verifying; using the molecular methods that is, by amplification of the internally transcribed spacer regions. By using the help of RAPD-PCR, 8Khan et al. (2007) studied diversity in various Aspergillus niger isolates sourc ed from pigeon pea fields .Several molecular techniques have been tested to classify different Aspergillus species like random amplification of polymorphic DNA (RAPD) (Yuan et al., 1995), the internal transcribed spacer (ITS) region (Kumeda and Asao, 1996; Henry et al., 2000; Kumeda and Asao, 2001; Rigo et al., 2002) and the aflatoxin gene cluster (Chang et al., 1995; Watson et al., 1999; Tominaga et al., 2006). In this study care was taken to choose the genomic DNA containing 18sRNA specifics primers that were helpful in amplifying medically important fungi. The genomic DNA containing 18s rRNA was the right candidate for detection of fungus as it is a mutli-copy gene which evolves slowly and is conserved among fungi. The present study proves that the genomic DNA containing 18s rRNA based PCR is suitable for probing large range of medically significant fungi owing to its higher level of analytical sensitivity and specificity. CONCLUSION: In this present study we had shown that molecular techniques are rapid and best for identification of fungi than the traditional morphological methods for early diagnosis and treatment of fungal infections. The goal of our study was to identify a practical, quick, cheap, method for the identification of A. fumigatus, the most common of the Aspergillus pathogens. REFERENCES: Arunachalam K, Arunachalam A, Tripathi RS, Pandey HN. 1997 – Dynamics of microbial population during the gradation phase of a selectively logged subtropical humid forest in north east India. Tropical Ecology 38, 333–341. Sirida Youngchim,1,2 Rachael Morris-Jones,1 Roderick J. Hay3 and Andrew J. Hamilton1 Production of melanin by Aspergillus fumigatus Journal of Medical Microbiology 2004, 53, 175–181. Domsch, K.H.,Gams W. and Anderson T.H. 1980.Compendium of soil fungi, vol 1.IHW-Verlag,Eching.,Raper,K.B.andFennel,D.I.1965.The genus Aspergillus (Baltimore: Williams Wilkins). Ferrer C, Colom F, Frases S, Mulet E, Abad JL, Alio JL:Detection and identification of fungal pathogens by PCR and by ITS2 and 5.8S ribosomal DNA in eye infections. J ClinMicrobiol 2001, 39(8): 2873-2879. Abraca ML, Bragulat G, Cabanes FJ 1994. Ochratoxin A production by strains of Aspergillus flavus var. niger . Appl. Environ. Microbiol. 60:2650-2652. Klich MA, Pitt JI 1988. Differentiation of Aspergillus flavus from Aspergillus parasiticus and other closely related species.Trans. Br.Mycol. Soc.91:99-108. Henson J, French R 1993. The polymerase chain reaction and plant disease diagnosis. Ann. Rev. Phytopathol.31:81-109. Khan MR, Anwer MA, Mohiddin FA 2007. Molecular diversity in Aspergillus isolates collected from pigeonpea field in Aligarh region.Environ. Biol. Conserv. 12:59-64. Godet F, Munaut F 2010. Molecular strategy for identification in Aspergillus section flavi. FEMS Microbiol. Lett. 304:157-168. Leema G, Kaliamurthy J, Geraldine J, Thomas PA 2010. Keratitis due to Aspergillus flavus: Clinical profile, molecular identification of fungal strains and detection of Aflotoxin production.Mol. Vision 6:843-854. Yuan G, Liu C, Chen C 1995. Differentiation of Aspergillus parasiticus from Aspergillus sojae by Random Amplification of Polymorphic DNA. Appl. Environ. Microbiol. 61:2384-2387. Kumeda Y, Asao T 2001. Heteroduplex panel analysis a novel method for genetic identification of Aspergillus section flavi strains. Appl. Environ. Microbiol. 67:4084-4090. Chang PK, Ehrlich KC, Bhatnagar JD, Cleveland TE 1995. Increased expression of Aspergillus parasiticus aflR, encoding a sequence specific DNA-binding protein, relieves nitrate inhibition of aflatoxin biosynthesis. Appl. Environ. Microbiol. 61:2372-2377.

Euthanasia Essay -- Mercy Killing Papers

Euthanasia The term 'Euthanasia' comes from the Greek word for 'easy death'. It is the one of the most public policy issues being debated about today. Formally called 'mercy killing', euthanasia is the act of purposely making or helping someone die, instead of allowing nature to take it's course. Basically euthanasia means killing in the name of compassion. Euthanasia, can be either 'voluntary', 'passive', or 'positive', Voluntary involves a request by the dying patient or their legal representative. Passive involves, doing nothing to prevent death - allowing someone to die. Positive involves taking deliberate action to cause a death. Euthanasia, at the moment is illegal throughout the world apart from in the State of Oregon, where there is a law specifically allowing doctors to prescribe lethal drugs for the purpose of euthanasia. In the Netherlands it is practised widely, although, in fact, it remains illegal. I believe that everyone has the right to choose how they live and die. Everyone deserves respect, freedom and the power to control their own destiny. Not everybody will have an easy death. Some terminal pain cannot be controlled, even with the best of care and the strongest of drugs. Other distressing symptoms, which come with diseases, such as sickness, no mobility, incontinence, breathlessness and fever cannot always be relieved. Pain is not always the issue - quality of life is too. Most people want to die with dignity, but some people may spend the last moments of their life, in a way which to them, is undignified. Having the right to control over their own life and death helps people keep human dignity in the face ... ...nimal is put to sleep. The owner is upset over the loss but they feel that they have done the right thing, by putting the pet out it's misery. I do not think we can look at human life in the same way however, as humans' are treated better than animals and have more respect. But what is better, letting someone suffer a prolonged and very painful life, or allowing him or her to die with dignity, in peace and without pain? This issue needs a lot of thought. Many people agree with voluntary euthanasia, many disagree but there is also a large amount of people undecided on the matter. The time will come when the Government and medical services will have to open their eyes to euthanasia, and there will be a lot of debate on the subject. Until then the euthanasia debate will continue to linger, like a terminal disease.